Prion Protein studied with Online Tools

Bovine Prion Protein analyzed with Online Interactive Tools

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Results of SAPS statistical study of bovine prion sequence
Results of MSF for recognizing bovine prion sequence repeats
Results of REPRO for recognizing protein sequence repeats
Results of a TagIdent search for proteins of similar size and isoelectric point
Results of 2D PAGE search for similarity
Results of Biocellerator homology search
Results of Signalp for prediction of the signal peptide cleavage sites

The online interactive tool, SAPS (Statistical Analysis of Protein Sequences) evaluates by statistical criteria a wide variety of protein sequence properties. Here's what the program returned when the amino acid sequence of bovine prion protein was run on August 27:

Bovine prion protein, 264 bases; molecular weight:  28,600; pIsoelectric=9.39
 
       1  MVKSHIGSWI LVLFVAMWSD VGLCKKRPKP GGGWNTGGSR YPGQGSPGGN RYPPQGGGGW 
      61  GQPHGGGWGQ PHGGGWGQPH GGGWGQPHGG GWGQPHGGGG WGQGGTHGQW NKPSKPKTNM 
     121  KHVAGAAAAG AVVGGLGGYM LGSAMSRPLI HFGSDYEDRY YRENMHRYPN QVYYRPVDQY 
     181  SNQNNFVHDC VNITVKEHTV TTTTKGENFT ETDIKMMERV VEQMCITQYQ RESQAYYQRG 
     241  ASVILFSSPP VILLISFLIF LIVG

Compositional Analysis, relative to a large dadabase of proteins, showed anomylous quantiles:
glycine-- 99%,tryptophan -- 95%, lysine and glutamate --5%

A	10( 3.8%)	C	3( 1.1%)	D-	6( 2.3%)	E	8( 3.0%)	F	7( 2.7%)
G++	51(19.3%)	H+	12( 4.5%)	I	11( 4.2%)	K	11( 4.2%)	L-	11( 4.2%)
M	9(3.4%)	N	11(4.2%)	P	18(6.8%)	Q	17(6.4%)	R	11(4.2%)
S	15(5.7%)	T	12(4.5%)	V	18(6.8%)	W+	10(3.8%)	Y	13(4.9%)

Charge and codon analysis of bovine prion protein:

+ charge	22(8.3%)	- charge	14(5.3%)	total charge	36(13.6%)	net + charge	8(3.0%)
FIKMNY	62(23.5%)	AGP+	79(29.9%)	ser-thr	27(10.2%)	hydrophobics	56(21.2%)

AGP: encoded by CCN, GCN, and GGN codons ... FIKMNY: encoded by AAN, AUN, UAN, and UUN codons

Charge distribution analysis:
Note SAPS fails to detect the pronounced positive and negative faces detected by NMR

 1  00+0000000 000000000- 0000+++0+0 000000000+ 0000000000 +000000000 
      61  0000000000 0000000000 0000000000 0000000000 0000000000 0+00+0+000 
     121  +000000000 0000000000 000000+000 0000-0--+0 0+-000+000 0000+00-00 
     181  00000000-0 00000+-000 0000+0-000 -0-0+00-+0 0-00000000 +-000000+0 
     241  0000000000 0000000000 0000

Positive charge clusters (cmin =  8/30 or 11/45 or 13/60):  none
Negative charge clusters (cmin =  6/30 or  8/45 or 10/60):  none
Mixed charge clusters (cmin = 11/30 or 15/45 or 18/60):  none

High scoring positive charge segments: none
High scoring negative charge segments: none
High scoring mixed charge segments:  none

Run count statistics:
  +  runs >=   3:   1, at   25
  -  runs >=   3:   0
  *  runs >=   3:   2, at   25;  157
  0  runs >=  37:   1,  at   52

High scoring hydrophobic segments:

 1) From  123 to  142:  length= 20, score=26.00  ** 
     123  VAGAAAAGAV VGGLGGYMLG
    L:  2(10.0%);  A:  6(30.0%);  G:  7(35.0%);  V:  3(15.0%);

 2) From  251 to  264:  length= 14, score=21.00  * 
     251  VILLISFLIF LIVG
    L:  4(28.6%);  V:  2(14.3%);  I:  4(28.6%);  F:  2(14.3%)

High scoring transmembrane segments:

 1) From  123 to  145:  length= 23, score=54.00  ** 
     123  VAGAAAAGAV VGGLGGYMLG SAM
    A:  7(30.4%);  G:  7(30.4%);  V:  3(13.0%);

 2) From  240 to  264:  length= 25, score=66.00  ** 
     240  GASVILFSSP PVILLISFLI FLIVG
    L:  5(20.0%);  S:  4(16.0%);  V:  3(12.0%);  I:  5(20.0%);
    F:  3(12.0%)

Spacing of Cysteines. NH2-23-C-165-C-34-C-39-COOH

Repeats:

Aligned matching blocks:

[  56-  70]   GGGGWGQPHGGGWGQ
[  97- 109]   GGGGWGQ__GGTHGQ

Simple tandem repeat: 

[  57-  64]    _GGGWGQPH
[  65-  72]    _GGGWGQPH
[  73-  80]    _GGGWGQPH
[  81-  88]    _GGGWGQPH
[  89-  96]    _GGGWGQPH
[  97- 103]    GGGGWGQ__
[ 104- 105]    GG

Repeats: using reduced 11-letter alphabet
(that lumps major hydrophobics LVIF, charged residues KR and ED, small residues AG, hydroxyl group residues ST, amide group residues NQ, and aromatics YW. )

Nothing significant found.

Homo-oligopeptides of any length:

1. Total number of amino acid multiplets:  26  (Expected range:   5-- 30)

2. Histogram of spacings between consecutive amino acid multiplets:
   (1-5) 11   (6-10) 8   (11-20) 5   (>=21) 3

3. Clusters of amino acid multiplets:  none

4. Significant specific amino acid altplets (e.g., RG, EAEAEA, etc.):

AA ........ Observed (Critical number)
Leu-Ile 7 (5) position: 10, 149, 244, 252, 254, 258, 261

Charge multiplets:

Nothing significant was found:


1. Total number of charge multiplets:   2  (Expected range:   0--  7)
   1 +plets (f+: 8.3%), 1 -plets (f-: 5.3%)

   Total number of charge altplets: 5 (Critical number: 9)

2. Histogram of spacings between consecutive charge multiplets:
   (1-5) 0   (6-10) 0   (11-20) 0   (>=21) 3

Periodic elements:

AMINO ACID ALPHABET 

Location	Period	Element		
  31- 102	 6	G.G...    	10	 4  	
  60-  99	 8	WGQPHGGG  	 5	 5 !
  90- 110	 3	G..       	 6	 4  	
 130- 145	 4	G...      	 4	 4  	

HYDROPHOBICITY ALPHABET {*= KRED; i= LVIF; 0}

Location .... 	Period	 ..... Element		
 251- 263	 1	LVIF-type         	12	 7 !	 1

Spacing analysis: The spacings between consecutive residues of the same type (all 20 amino acids, + and - charge, and combined charge *) are evaluated for signifi- cantly large or small maximal and minimal spacings. The output is ordered by the beginning point of the significant spacing. Entries are identified by the residue type, spacing (number of amino acids between the identified positions), rank of the displayed spacing (e.g., 50 alanines in the sequence induce 51 spacings, ranked by decreasing length from 1 to 51), and p-value (probability of exceeding the displayed spacing).. If the first maximal spacing (rank 1) of a residue is significantly large or small, then also the second maximal spacing (rank 2) is evaluated. Large maximal and small minimal spacings indicate clustering effects, whereas small max- imal and large minimal spacings indicate excessive evenness in the distri- bution of the residues.

Location (Quartile) Spacing     Rank       P-value   Interpretation

   0- 157  (2.)     E( 157)E     1 of   9   0.0056   large  1. maximal spacing
  10- 150  (2.)     I( 140)I     1 of  12   0.0023   large maximal spacing
  20- 155  (2.)     -( 135)-     1 of  15   0.0005   large maximal spacing
  21- 123  (2.)     V( 102)V     1 of  19   0.0020   large maximal spacing
  34-  60  (1.)     W(  26)W     2 of  11   0.9959   small  2. maximal spacing
  51- 112  (2.)     *(  61)*     1 of  37   0.0013   large maximal spacing
 110- 265  (3.)     W( 155)W     1 of  11   0.0012   large  1. maximal spacing
 153- 206  (3.)     G(  53)G     1 of  52   0.0001   large  1. maximal spacing
 163- 197  (3.)     E(  34)E     2 of   9   0.9694   small  2. maximal spacing
 206- 240  (4.)     G(  34)G     2 of  52   0.0000   large  2. maximal spacing

Multiple alignment of repeat sets:

64_73           GGTNWG..QP .....HP... .........
74_83           GGSNWG..QP .....HP... .........
84_92           GGSSWG..QP .....H.... .........
93_100         GGSNWG..QG .......... .........
101_129       GYNKWKPDKP KTNLKHVAGA AAAGAVVGG

57_66           GWGH...... .P........ QGGGT..... ....
67_76           NWGQ...... .P........ HPGGS..... ....
77_86           NWGQ...... .P........ HPGGS..... ....
87_95           SWGQ...... .P........ HGGS...... ....
96_129         NWGQGGYNKW KPDKPKTNLK HVAGAAAAGA VVGG

56_75           PGWGHPQGGG TNWG..QP.. ...HPGG... .......
76_94           SNWGQPHPGG SSWG..QP.. ...HGG.... .......
95_129         SNWGQ..GGY NKWKPDKPKT NLKHVAGAAA AGAVVGG

4_16            IQLGYWILVL FIV
245_257     VTLLFLSFLI FLI

18_47           WSDLGLCKKP KPRPGGG..W NSGGSNRY.P GQP
78_110         WGQPHPGGSS WGQPHGGSNW GQGGYNKWKP DKP

29_70           PRPGGGWNSG GSNRYPGQPG SPGGNRYPGW GHPQGGGTNW GQ
71_99           PHPG...... GSN..WGQP. HPGGS...SW GQPHGG.SNW GQ

37_45           SGGSNRYPG
49_57           SPGGNRYPG 
42_57           RYPGQPGSPG GNRYPG
161_176       RYPNQVMYRP IDQYSS

139_149       MSRPVIHFGN E
167_177        MYRPIDQYSS Q 

143_167        VIHFGNEYED ...RYYRENQ YRYPNQVM
220_247        ITQYQAEYEA AAQRAYNMAF FSAPPVTL

This REPRO output is a result of a sequence self comparison. Just the subsequences with highest similarity scores (top non-overlapping pairwise alignments) are listed (numbered sequentially under 'repeat' followed by residue length, score and score per residue). This is followed by a list of repeat sets deduced from the top alignments (called 'repeat set').

The sets are ordered on the numbers of repeating fragments they obtain. Finally, for each set the repeat segments are multiply aligned. The repeat fragments in different sets can be similar but reflect different sequence site ranges (i.e. the repeats are shifted with one to a few residues).


repeat    1   length = 20   score =      89.00   mean score =       4.45

   56  PGWGHPQGGG TNWGQPHPGG   75
         || |  ||   ||||| | 
   76  SNWGQPHPGG SSWGQPHGGS   95
-
repeat    2   length = 43   score =      82.00   mean score =       1.91

   29  PRPGGG-WNS GGSNRYPGQP GSPGGNRYPG WGHPQGGGTN WGQ   70
       | |||  |          |||   |||      || |  || | |||
   71  PHPGGSNW-- -------GQP -HPGG---SS WGQPH-GGSN WGQ   99
-
repeat    3   length = 10   score =      58.00   mean score =       5.80

   67  NWGQPHPGGS   76
       ||||||||||
   77  NWGQPHPGGS   86
-
repeat    4   length = 33   score =      47.00   mean score =       1.42

   18  WSD--LGLCK KPKPRPGGGW NSGGSNRY-P GQP   47
       |     |       |  |  |   || |   |   |
   78  WGQPHPGGSS WGQPHGGSNW GQGGYNKWKP DKP  110
-
repeat    5   length = 10   score =      45.00   mean score =       4.50

   77  NWGQPHPGGS   86
        ||||| |  
   87  SWGQPHGGSN   96
-
repeat    6   length = 18   score =      42.00   mean score =       2.33

   74  GGSNWGQPHP GGSSWGQP   91
       |||||||    |   |   
   93  GGSNWGQ--G GYNKWKPD  108
-
repeat    7   length = 39   score =      39.00   mean score =       1.00

   56  PGWGHPQGGG TNWGQPHPGG SNWGQPHPGG SSWGQPHGG   94
         ||  |||    |    |           |     |   ||
   95  SNWG--QGGY NKWKPDKPKT NL--KHVAGA AAAGAVVGG  129
-
repeat    8   length = 8   score =      38.00   mean score =       4.75

   84  GGSSWGQP   91
       ||| ||| 
   93  GGSNWGQG  100
-
repeat    9   length = 28   score =      37.00   mean score =       1.32

  143  VIHFGNEYE- --DRYYRENQ YRYPNQVM  167
             |||     | |        |    
  220  ITQYQAEYEA AAQRAYNMAF FSAPPVTL  247
-
repeat   10   length = 9   score =      36.00   mean score =       4.00

   37  SGGSNRYPG   45
       | | |||||
   49  SPGGNRYPG   57
-
repeat   11   length = 11   score =      31.00   mean score =       2.82

  139  MSRPVIHFGN E  149
       | ||        
  167  MYRPIDQYSS Q  177
-
repeat   12   length = 16   score =      30.00   mean score =       1.88

   42  RYPGQPGSPG GNRYPG   57
       ||| |         |  
  161  RYPNQVMYRP IDQYSS  176
-
repeat   13   length = 13   score =      30.00   mean score =       2.31

    4  IQLGYWILVL FIV   16
         |        |  
  245  VTLLFLSFLI FLI  257

-
Sequence fragments derived from graph clustering:

repeat set: 5 fragments
64_73
GGTNWGQPHP*

74_83
GGSNWGQPHP*

84_92
GGSSWGQPH*

93_100
GGSNWGQG*

101_129
GYNKWKPDKPKTNLKHVAGAAAAGAVVGG*
-
repeat set: 5 fragments
57_66
GWGHPQGGGT*

67_76
NWGQPHPGGS*

77_86
NWGQPHPGGS*

87_95
SWGQPHGGS*

96_129
NWGQGGYNKWKPDKPKTNLKHVAGAAAAGAVVGG*
-
repeat set: 3 fragments
56_75
PGWGHPQGGGTNWGQPHPGG*

76_94
SNWGQPHPGGSSWGQPHGG*

95_129
SNWGQGGYNKWKPDKPKTNLKHVAGAAAAGAVVGG*
-
repeat set: 2 fragments
4_16
IQLGYWILVLFIV*

245_257
VTLLFLSFLIFLI*
-
repeat set: 2 fragments
18_47
WSDLGLCKKPKPRPGGGWNSGGSNRYPGQP*

78_110
WGQPHPGGSSWGQPHGGSNWGQGGYNKWKPDKP*
-
repeat set: 2 fragments
29_70
PRPGGGWNSGGSNRYPGQPGSPGGNRYPGWGHPQGGGTNWGQ*

71_99
PHPGGSNWGQPHPGGSSWGQPHGGSNWGQ*
-
repeat set: 2 fragments
37_45
SGGSNRYPG*

49_57
SPGGNRYPG*
-
repeat set: 2 fragments
42_57
RYPGQPGSPGGNRYPG*

161_176
RYPNQVMYRPIDQYSS*
-
repeat set: 2 fragments
139_149
MSRPVIHFGNE*

167_177
MYRPIDQYSSQ*
-
repeat set: 2 fragments
143_167
VIHFGNEYEDRYYRENQYRYPNQVM*

220_247
ITQYQAEYEAAAQRAYNMAFFSAPPVTL*
-

Here are the results of the TagIdent Search. This looks for proteins close to bovine prion in molecular weight and isoelecric point. It is a way of finding homlogous or similar proteins. The first 40 amino acids are also provided.

Search performed with following values:
        pI =            9.4
        Mw =            25000
        delta-pI =      0.3
        delta-Mw =      5000
        OS or OC =      MAMMALIA
---------------------
154 proteins found (only a few are retained here)
        ABME_RABIT      (P47855)
                [pI = 9.17; Mw = 27719.0]
                MASEK GPSNK DYTLR RRIEP WEFEV FFDPQ ELRKE ACLLY

        ACRO_PIG        (P08001)
                [pI = 9.66; Mw = 43837.24]
                [pI = 8.95; Mw = 2611]
                [pI = 9.35; Mw = 2790]
                MLPTAVLLVLAVSVAARDNATCDGPCGLRFRQKLESGMRV

        ARL4_HUMAN      (P40617)
                [pI = 9.26; Mw = 22615.0]
                MGNGLSDQTSILSNLPSFQSFHIVILGLDCAGKTTVLYRL

        ARL4_RAT        (P41275)
                [pI = 9.26; Mw = 22588.0]
                MGNGLSDQTSILSSLPSFQSFHIVILGLDCAGKTTVLYRL

        ASH2_RAT        (P19360)
                [pI = 9.32; Mw = 27694.7]
                MESHFNWYGVPRLQKASDACPRESCSSALPEAREGANVHF

        ATP6_HALGR      (P38591)
                [pI = 9.30; Mw = 24862.6]
                MNENLFASFTTPTMMGLPIVILIVLFPSILFPSPDRLINN

        ATP6_PHOVI      (Q00521)
                [pI = 9.69; Mw = 24793.5]
                MNENLFASFATPTMMGLPIVILIVLFPSILFPSPDRLINN

        ATP6_RAT        (P05504)
                [pI = 9.60; Mw = 25050.1]
                MNENLFASFITPTMMGLPIVVTIIMFPSILFPSSERLISN

        ATPF_BOVIN      (P13619)
                [pI = 9.14; Mw = 24668.5]
                PVPPLPEHGGKVRFGLIPEEFFQFLYPKTGVTGPYVLGTG

                MIMSSYLMDSNYIDPKFPPCEEYSQNSYIPEHSPEYYGRT

        PRIO_BOVIN      (P10279)
                [pI = 9.49; Mw = 25939.9]
                MVKSHIGSWILVLFVAMWSDVGLCKKRPKPGGGWNTGGSR

        PRIO_HUMAN      (P04156)
                [pI = 9.39; Mw = 25235.2]
                MANLGCWMLVLFVATWSDLGLCKKRPKPGGWNTGGSRYPG

        PRIO_MACFA      (P40254)
                [pI = 9.45; Mw = 25250.3]
                MANLGCWMLVLFVATWSDLGLCKKRPKPGGWNTGGSRYPG

        PRIO_MESAU      (P04273)
                [pI = 9.58; Mw = 22973.4]
                MANLSYWLLALFVAMWTDVGLCKKRPKPGGWNTGGSRYPG

        PRIO_MOUSE      (P04925)
                [pI = 9.56; Mw = 25477.4]
                MANLGYWLLALFVTMWTDVGLCKKRPKPGGWNTGGSRYPG

        PRIO_MUSVI      (P40244)
                [pI = 9.45; Mw = 25074.1]
                MVKSHIGSWLLVLFVATWSDIGFCKKRPKPGGGWNTGGSR

        SPC4_RAT        (P42667)
                [pI = 9.15; Mw = 20599.1]
                MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLM

       ; Mw = 25791.3]
                MGVFCLGPWGLGRKLRTPGKGPLQLLSRLCGDHLQAIPAK

        VATD_BOVIN      (P39942)
                [pI = 9.44; Mw = 28333.6]
                MSGKDRIEIFPSRMAQTIMKARLKGAQTGRNLLKKKSDAL

        VATE_MOUSE      (P50518)
                [pI = 9.28; Mw = 26587.8]

Here are the proteins most likely to be confused with bovine prion on 2D polyacrylamide gel electrophoresis (molecular weight and pI).

  1     8 PRIO_HUMAN   9.39    25235 PRION PROTEIN.
   2    73 C1QA_HUMAN   9.34    23688 COMPLEMENT C1Q SUBCOMPONENT, A CHAIN.
   3   193 HXD4_HUMAN   9.44    27895 HOMEOBOX PROTEIN HOX-D4 (HOX-4B) (HOX-5
   4   230 CAP7_HUMAN   9.53    24051 AZUROCIDIN.
   5   231 MCP1_HUMAN   9.60    25030 CHYMASE.
   6   231 UNG1_HUMAN   9.28    25791 URACIL-DNA GLYCOSYLASE.
   7   257 C24A_HUMAN   9.61    20827 CYTOCHROME B-245 LIGHT CHAIN (P22
   8   278 MYP0_HUMAN   9.57    24763 MYELIN P0 PROTEIN.
   9   286 RS3_HUMAN    9.68    26688 40S RIBOSOMAL PROTEIN S3.
  10   287 BTF3_HUMAN   9.41    22168 BTF3A.
  11   321 OC3B_HUMAN   9.39    30084 OCTAMER-BINDING TRANSCRIPTION FACTOR 3B 
  12   327 HXA5_HUMAN   9.32    29359 HOMEOBOX PROTEIN HOX-A5 (HOX-1C).
  13   343 CD28_HUMAN   9.39    23085 T-CELL-SPECIFIC SURFACE GLYCOPROTEINCD28
  14   344 RU2B_HUMAN   9.72    25486 U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B".
  15   345 TCF1_HUMAN   9.62    30264 T-CELL-SPECIFIC TRANSCRIPTION FACTOR 1 
  16   345 T2FB_HUMAN   9.24    28380 TRANSCRIPTION INITIATION FACTOR IIF,
  17   352 CDX1_HUMAN   9.58    28124 HOMEOBOX PROTEIN CDX-1.
  18   355 G11_HUMAN    9.30    28885 G11 PROTEIN.
  19   356 S5A2_HUMAN   9.47    28393 3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 2 
  20   363 HXC5_HUMAN   9.57    24976 HOMEOBOX PROTEIN HOX-C5 (HOX-3D) (CP11)

Biocellerator homology searches

* Maruspial sequence run against databases on theory that it might be more conservative than eutherian prion proteins, hence have more easily recognized homology. The search engines returned lots of prion proteins; below them some high glycine proteins returned at much weaker homology:

...
rat             prion      ......                                               914
chicken    prion ......                                                    443
fruit fly    hnRNP  .....                                                 151
human   fibrinogen alpha-1 chain precursor       137

...

* Run with chicken prion protein on the theory that it might be more closely related to older homologous proteins.

...
rat                     prion         392
slime mold        annexin     204
human               annexin     147
...

* Test against fragment of chicken hexapeptide repeat: PSGGGWGAGS HRQPSYPRQP GYPHN PGYPH is interesting because other prions are not near neighbors.

...
slime mold        annexin
human               coup transcription
...

Proposed but not done yet: Run homology search on prion anti-gene, the large open reading frame of unknown function found on the anti-sense gene in all animals except mink and marsupial, on the theory that a duplicated anti-gene could have had the original function and the prion gene evolved from it later. See:

Nature 362: 213-4 (1993) "An anti-prion protein?" [letter], Moser M; Oesch B; Bueler H 
Nature 351: 106 (1991) "Anticipating the anti-prion protein?"[letter], Goldgaber D 
Gene 159: 181-186 1995 Windl, O et al

Using neural networks trained on eukaryotic signal peptide data.

prion: first 43 residues of consensus sequence

# pos  aa    C       S       Y
    1   M   0.011   0.919   0.000
    2   V   0.011   0.942   0.000
    3   K   0.011   0.945   0.000
    4   S   0.011   0.945   0.000
    5   H   0.013   0.949   0.000
    6   I   0.010   0.940   0.000
    7   G   0.012   0.928   0.000
    8   S   0.012   0.962   0.000
    9   W   0.012   0.950   0.000
   10   L   0.011   0.971   0.005
   11   L   0.013   0.982   0.016
   12   V   0.012   0.981   0.022
   13   L   0.012   0.978   0.034
   14   F   0.014   0.966   0.046
   15   V   0.013   0.920   0.055
   16   A   0.017   0.929   0.071
   17   T   0.033   0.910   0.111
   18   W   0.042   0.920   0.138
   19   S   0.047   0.907   0.157
   20   D   0.201   0.832   0.347
   21   L   0.068   0.820   0.212
   22   G   0.136   0.799   0.312
   23   L   0.111   0.436   0.292
   24   C   0.074   0.395   0.236
   25   K   0.656   0.153   0.694
   26   K   0.075   0.129   0.224
   27   R   0.106   0.098   0.255
   28   P   0.016   0.079   0.094
   29   K   0.060   0.057   0.167
   30   P   0.013   0.049   0.071
   31   G   0.020   0.041   0.080
   32   G   0.025   0.034   0.078
   33   G   0.017   0.035   0.055
   34   W   0.050   0.044   0.072
   35   N   0.017   0.030   0.035
   36   T   0.014   0.028   0.022
   37   G   0.013   0.029   0.017
   38   G   0.011   0.029   0.012
   39   S   0.014   0.036   0.009
   40   R   0.014   0.031   0.005
   41   Y   0.017   0.030   0.000
   42   P   0.012   0.037   0.000
   43   G   0.011   0.037   0.000
                                 Signal peptide?
C max = 0.656 at pos. 25
S max = 0.982 at pos. 11         YES (cutoff = 0.87)
Y max = 0.694 at pos. 25         YES (cutoff = 0.31)
S mean (from 1 to 24) = 0.849    YES (cutoff = 0.50)
Most likely cleavage site between pos. 24 and 25